What question did this study set out to answer?

To identify and characterize the protein kinase complex associated with human cyclin E during the G1 phase of the cell cycle.

September 18, 1992Science

Formation and Activation of a Cyclin E-cdk2 Complex During the G 1 Phase of the Human Cell Cycle

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Authors

AKAndrew KoffKettering University AGAntonio GiordanoUniversity of Siena DDDipty DesaiUniversity of California, San Francisco

Discussion

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Implication

In vitro study reveals cyclin E forms an active complex with Cdk2 peaking during the G1 phase in human cells, suggesting a universal eukaryotic mechanism regulating cell proliferation.

Key Points

To identify and characterize the protein kinase complex associated with human cyclin E during the G1 phase of the cell cycle.
Analyzed human cell lines across distinct cell cycle stages, differentiation states, and serum withdrawal conditions.
Measured cyclin E protein abundance, complex formation, and associated kinase activity relative to cyclin-dependent kinase 2 (Cdk2) and cyclin A.
Cyclin E associated primarily with Cdk2 in human cells, with both cyclin E abundance and cyclin E-Cdk2 complex formation reaching maximum levels in the G1 phase.
Cyclin E-associated kinase activity peaked during G1 prior to cyclin A appearance and declined significantly upon cell cycle exit induced by differentiation or serum starvation.

Cite This Study

Koff et al. (1992) studied this question.

synapsesocial.com/papers/6a1ffb76d4e6d3589704c2e9 https://doi.org/10.1126/science.1388288

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September 18, 1992Science

Formation and Activation of a Cyclin E-cdk2 Complex During the G 1 Phase of the Human Cell Cycle

View Full Paper

Ask AI

Bookmark

Authors

AKAndrew KoffKettering University AGAntonio GiordanoUniversity of Siena DDDipty DesaiUniversity of California, San Francisco

Discussion

Member takes

Implication

In vitro study reveals cyclin E forms an active complex with Cdk2 peaking during the G1 phase in human cells, suggesting a universal eukaryotic mechanism regulating cell proliferation.

Key Points

To identify and characterize the protein kinase complex associated with human cyclin E during the G1 phase of the cell cycle.
Analyzed human cell lines across distinct cell cycle stages, differentiation states, and serum withdrawal conditions.
Measured cyclin E protein abundance, complex formation, and associated kinase activity relative to cyclin-dependent kinase 2 (Cdk2) and cyclin A.
Cyclin E associated primarily with Cdk2 in human cells, with both cyclin E abundance and cyclin E-Cdk2 complex formation reaching maximum levels in the G1 phase.
Cyclin E-associated kinase activity peaked during G1 prior to cyclin A appearance and declined significantly upon cell cycle exit induced by differentiation or serum starvation.

Cite This Study

Koff et al. (1992) studied this question.

synapsesocial.com/papers/6a1ffb76d4e6d3589704c2e9 https://doi.org/10.1126/science.1388288

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