What question did this study set out to answer?

To identify and characterize the protein kinase complex associated with human cyclin E during the G1 phase of the cell cycle.

September 18, 1992Science

Formation and Activation of a Cyclin E-cdk2 Complex During the G 1 Phase of the Human Cell Cycle

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Authors

AKAndrew KoffKettering University AGAntonio GiordanoUniversity of Siena DDDipty DesaiUniversity of California, San Francisco

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Implication

In vitro study reveals cyclin E forms an active complex with Cdk2 peaking during the G1 phase in human cells, suggesting a universal eukaryotic mechanism regulating cell proliferation.

Key Points

To identify and characterize the protein kinase complex associated with human cyclin E during the G1 phase of the cell cycle.
Analyzed human cell lines across distinct cell cycle stages, differentiation states, and serum withdrawal conditions.
Measured cyclin E protein abundance, complex formation, and associated kinase activity relative to cyclin-dependent kinase 2 (Cdk2) and cyclin A.
Cyclin E associated primarily with Cdk2 in human cells, with both cyclin E abundance and cyclin E-Cdk2 complex formation reaching maximum levels in the G1 phase.
Cyclin E-associated kinase activity peaked during G1 prior to cyclin A appearance and declined significantly upon cell cycle exit induced by differentiation or serum starvation.

Cite This Study

Koff et al. (1992) studied this question.

synapsesocial.com/papers/6a1ffb76d4e6d3589704c2e9 https://doi.org/10.1126/science.1388288

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Implication

In vitro study reveals cyclin E forms an active complex with Cdk2 peaking during the G1 phase in human cells, suggesting a universal eukaryotic mechanism regulating cell proliferation.

Formation and Activation of a Cyclin E-cdk2 Complex During the G 1 Phase of the Human Cell Cycle

Discussion

Member takes

Implication

Key Points

Cite This Study

Formation and Activation of a Cyclin E-cdk2 Complex During the G 1 Phase of the Human Cell Cycle

Discussion

Member takes

Implication

Key Points

Cite This Study

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