What question did this study set out to answer?

To review the historical discoveries establishing that Cdc2 and cyclin form a stoichiometric heterodimer essential for MPF kinase activity and cell cycle entry into mitosis.

June 15, 2002Journal of Cell Science

From Cdc2 to Cdk1: when did the cell cycle kinase join its cyclin partner?

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Authors

MDMarcel DoréeCentre National de la Recherche Scientifique THTim HuntTwitter (United States)

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Implication

Historical review traces the molecular identification of maturation-promoting factor as a Cdc2-cyclin complex, highlighting how Cdc2 became redefined as Cdk1.

Key Points

To review the historical discoveries establishing that Cdc2 and cyclin form a stoichiometric heterodimer essential for MPF kinase activity and cell cycle entry into mitosis.
Synthesized historical genetic analysis from fission yeast with physiological and biochemical findings in marine invertebrates and amphibian oocytes.
Tracked the identification of MPF from its initial phenotypic observation through protein purification and microsequencing.
Purification and sequencing of starfish MPF demonstrated that the active complex is a heterodimer composed of one Cdc2 molecule and one cyclin B molecule.
Evidence confirmed that cyclin association is strictly required for the kinase activity of Cdc2, establishing Cdc2 as Cdk1 across eukaryotes.

Cite This Study

Dorée et al. (2002) studied this question.

synapsesocial.com/papers/6a0cbd5dd466daa8c5f2619e https://doi.org/10.1242/jcs.115.12.2461

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Implication

Historical review traces the molecular identification of maturation-promoting factor as a Cdc2-cyclin complex, highlighting how Cdc2 became redefined as Cdk1.

From Cdc2 to Cdk1: when did the cell cycle kinase join its cyclin partner?

Discussion

Member takes

Implication

Key Points

Cite This Study

From Cdc2 to Cdk1: when did the cell cycle kinase join its cyclin partner?

Discussion

Member takes

Implication

Key Points

Cite This Study

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