What question did this study set out to answer?

To determine how mammalian cells maintain constant steady-state concentrations of the cdc2 catalytic subunit throughout the cell cycle.

April 1, 1992Proceedings of the National Academy of SciencesOpen Access

Coordinated synthesis and degradation of cdc2 in the mammalian cell cycle.

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Authors

PWPeter J. WelchCedars-Sinai Medical Center JWJiemin WangScience Foundation Ireland

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Implication

Cellular study uncovers coordinated synthesis and degradation governing cdc2 levels during mammalian cell cycles, indicating active protein turnover regulates division.

Key Points

To determine how mammalian cells maintain constant steady-state concentrations of the cdc2 catalytic subunit throughout the cell cycle.
Tracked cdc2 gene transcription and mRNA degradation rates across mitotic and interphase stages.
Quantified cdc2 translation and protein half-life during the G1 phase and following the G1/S transition under growth factor stimulation.
Measured the proportion of newly translated cdc2 that complexes with cyclin to form M phase-promoting factor.
cdc2 gene transcription increases 12-fold at the G1/S transition, whereas translation shuts off at mitotic onset and mRNA degrades rapidly after mitosis.
Protein half-life drops from 18 hours during G1 phase to 7.5 hours following G1/S transition, with newly synthesized cdc2 comprising 75% to 85% of total cdc2 by the end of interphase.
Greater than 75% of newly generated cdc2 forms complexes with cyclin to function within M phase-promoting factor.

Cite This Study

Welch et al. (1992) studied this question.

synapsesocial.com/papers/6a7dce9afa78bd2571186e3d https://doi.org/10.1073/pnas.89.7.3093

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Implication

Cellular study uncovers coordinated synthesis and degradation governing cdc2 levels during mammalian cell cycles, indicating active protein turnover regulates division.

Coordinated synthesis and degradation of cdc2 in the mammalian cell cycle.

Discussion

Member takes

Implication

Key Points

Cite This Study

Coordinated synthesis and degradation of cdc2 in the mammalian cell cycle.

Discussion

Member takes

Implication

Key Points

Cite This Study

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