What question did this study set out to answer?

To characterize the immunological, cytogenetic, and cytochemical properties of the NALM-6-M1 cell line derived from a patient with relapsed acute lymphoblastic leukemia.

February 15, 1979International Journal of Cancer

Characterization of a leukemic cell line of the pre‐B phenotype

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Authors

RHRichard L. HurwitzCleveland State University JHJohn HozierUniversity of New Mexico TLTucker W. LeBienUniversity of Minnesota

Discussion

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Implication

In vitro characterization reveals cytoplasmic immunoglobulin expression and 5q deletion in a leukemic cell line, indicating developmental arrest at the pre-B cell stage.

Key Points

To characterize the immunological, cytogenetic, and cytochemical properties of the NALM-6-M1 cell line derived from a patient with relapsed acute lymphoblastic leukemia.
Cultured the NALM-6-M1 cell line from the peripheral blood of a 19-year-old male with relapsed non-T, non-B acute lymphoblastic leukemia.
Evaluated surface and cytoplasmic immunoglobulin markers, receptor profiles, mixed leukocyte culture responsiveness, cytochemical staining, and karyotypic abnormalities.
More than 90% of cells expressed cytoplasmic immunoglobulin mu heavy and lambda light chains, but lacked surface immunoglobulins and complement receptors.
Cytogenetic analysis revealed a deleted long arm of chromosome 5 (5q−) and a marker Y chromosome in approximately 90% of cells, while 10% showed additional material on chromosome 12.

Cite This Study

Hurwitz et al. (1979) studied this question.

synapsesocial.com/papers/6a2003bf7110a651dc04dab1 https://doi.org/10.1002/ijc.2910230206

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Implication

In vitro characterization reveals cytoplasmic immunoglobulin expression and 5q deletion in a leukemic cell line, indicating developmental arrest at the pre-B cell stage.

Characterization of a leukemic cell line of the pre‐B phenotype

Discussion

Member takes

Implication

Key Points

Cite This Study

Characterization of a leukemic cell line of the pre‐B phenotype

Discussion

Member takes

Implication

Key Points

Cite This Study

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