Key result
A method using enzymatic treatment and paramagnetic beads successfully isolated human cardiac macro- and microvascular endothelial cells with high purity (<2% nonendothelial cells).
Population
Human macro- and microvascular endothelial cells isolated from cardiac muscle preparations and epicardial…
Design
Preclinical
Authors
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No immediate clinical applications; extends in vitro tools for human cardiac endothelial research.
The established method allows for the selective isolation and culture of human macro- and microvascular cardiac endothelial cells with high purity, providing a valuable tool to investigate endothelial function in human hearts.
Gräfe et al. (1994) studied this question. Isolation and culture of macro- and microvascular endothelial cells was evaluated on Endothelial cell purity and functional heterogeneity. A method using enzymatic treatment and paramagnetic beads successfully isolated human cardiac macro- and microvascular endothelial cells with high purity (<2% nonendothelial cells).
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