Key result
Ramipril and losartan prevented the significant increase in systolic blood pressure induced by reduced renal mass in rats, though TGF-beta 1 mRNA levels were increased threefold (P<0.05) in all RRM groups.
Why the study?
Does ramipril or losartan prevent hypertension and alter AT1 gene expression in rats with reduced renal mass?
Does ramipril or losartan prevent hypertension and alter AT1 gene expression in rats with reduced renal mass?
p-value: p=<0.05
The development of reduced renal mass hypertension is ANG II dependent but not mediated by AT1 gene expression, which is downregulated by local injury.
Supports ANG II dependence of reduced renal mass hypertension in rats; leaves open translation to human CKD and AT1 mediation.
To investigate the role of angiotensin II (ANG II) in the development of hypertension induced by reduced renal mass (RRM) and the gene expression of ANG II type 1 (AT1) receptors in the remnant renal tissue, four groups of rats were given 1% NaCl in water and subjected to RRM, RRM+ ramipril, RRM+ losartan, or sham surgery (control). Tail-cuff systolic blood pressure was significantly higher in RRM rats than in the other three groups. Northern blot showed that AT1 gene expression was significantly decreased in RRM, RRM + ramipril, or RRM + losartan vs. control. There was no significant difference among the three RRM groups. Renal transforming growth factor-beta 1 (TGF-beta 1) mRNA levels were increased threefold (P < 0.05) in RRM, RRM+ ramipril, and RRM+ losartan vs. control. There was no significant difference among the three RRM groups. We conclude that the development of RRM hypertension is ANG II dependent but not mediated by AT1 gene expression. RRM downregulates AT1 mRNA and upregulates TGF-beta 1 mRNA in the remnant renal tissue, regardless of blood pressure or plasma levels of ANG II, suggesting that these gene responses are triggered by an effect of local injury.
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Wang et al. (1996) studied Hypertension induced by reduced renal mass. Ramipril or losartan vs. RRM alone and sham surgery was evaluated on Tail-cuff systolic blood pressure and gene expression (AT1, TGF-beta 1) (p=<0.05). Ramipril and losartan prevented the significant increase in systolic blood pressure induced by reduced renal mass in rats, though TGF-beta 1 mRNA levels were increased threefold (P<0.05) in all RRM groups.
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