What is the clinical evidence from this study?

Study design: Other. Population: Ventricular myocardium physiology. Intervention: Activation of voltage-gated Na channels. Primary outcome: Transient activation of Na/K pump current (I(p)).

What question did this study set out to answer?

This research aims to determine if sodium influx through voltage-gated sodium channels increases sub-sarcolemmal sodium levels and activates the Na/K pump;

March 15, 2003Cardiovascular ResearchOpen Access

Is there a transient rise in sub-sarcolemmal Na and activation of Na/K pump current following activation of INa in ventricular myocardium?

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Key result

Activation of Na influx via voltage-gated Na channels did not cause a transient activation of Na/K pump current or change the sub-sarcolemmal fuzzy-space Na concentration.

Population

Single guinea-pig ventricular myocytes and intact rabbit papillary muscles

Design

Preclinical

Authors

BSBernard L. SilvermanOffice of Chief Medical Examiner

Discussion

Member takes

Implication

Challenges fuzzy-space Na models of pump activation; hypothesis-generating for Na handling in ventricular myocardium.

Key Points

This research aims to determine if sodium influx through voltage-gated sodium channels increases sub-sarcolemmal sodium levels and activates the Na/K pump;
Investigated Na/K pump activity in voltage-clamped single guinea pig ventricular myocytes.
Measured sub-sarcolemmal sodium gradients in rabbit papillary muscles using electron probe X-ray microanalysis.
Examined transient Na/K pump activation following voltage-gated sodium channel activation.
Observed maintained sub-sarcolemmal sodium levels in ventricular myocardium.
No transient Na/K pump activation detected after sodium influx during voltage clamp experiments.
Sub-sarcolemmal sodium gradient remained stable throughout the contractile cycle.

Structured PICO

Population

Single guinea-pig ventricular myocytes and intact rabbit papillary muscles

Exposure

Activation of Na influx via voltage-gated Na channels using voltage clamp steps

Outcome

Sub-sarcolemmal [Na] and transient activation of Na/K pump current (I(p))surrogate

The study provides evidence for a maintained sub-sarcolemmal sodium gradient in ventricular myocardium that remains stable during the contractile cycle and immediately following sodium influx.

Cite This Study

Bernard L. Silverman (2003) studied Ventricular myocardium physiology. Activation of voltage-gated Na channels was evaluated on Transient activation of Na/K pump current (I(p)). Activation of Na influx via voltage-gated Na channels did not cause a transient activation of Na/K pump current or change the sub-sarcolemmal fuzzy-space Na concentration.

synapsesocial.com/papers/6a2044e6b64142f67e45f3da https://doi.org/10.1016/s0008-6363(02)00645-4

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Is there a transient rise in sub-sarcolemmal Na and activation of Na/K pump current following activation of INa in ventricular myocardium?

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Is there a transient rise in sub-sarcolemmal Na and activation of Na/K pump current following activation of INa in ventricular myocardium?

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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