Continuous Measurement of Cerebral Cortical Blood Flow by Laser—Doppler Flowmetry in a Rat Stroke Model
Population
Wistar and spontaneously hypertensive rats subjected to permanent occlusion of the middle cerebral and…
Comparison
Laser-Doppler flowmetry (LDF) vs Autoradiographic measurement with…
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Relative LDF may track percent rCBF changes in rat focal ischemia; leaves open absolute quantification and clinical translation.
Key Points
- To evaluate the accuracy of laser-Doppler flowmetry (LDF) in measuring regional cerebral blood flow (rCBF) after focal ischemia in rats.
- Wistar and spontaneously hypertensive rats (n=19) underwent permanent occlusion of major arteries.
- Absolute rCBF measured with autoradiographic [14C]iodoantipyrine; compared to rCBF measured using laser-Doppler flowmetry.
- Percent change in rCBF from baseline compared between both measurement methods.
- LDF measurements of absolute rCBF correlated poorly with [14C]iodoantipyrine (r=0.54).
- Relative LDF readings showed strong correlation with percent change in [14C]iodoantipyrine measurements (r=0.91).
- LDF is not accurate for absolute rCBF but accurately measures changes in rCBF after ischemia.
Structured PICO
P
Population
Wistar and spontaneously hypertensive rats (SHR, n = 19) subjected to permanent occlusion of the middle cerebral and common carotid arteries
I
Intervention
Laser-Doppler flowmetry (LDF)
C
Comparator
Autoradiographic measurement with [14C]iodoantipyrine
O
Outcome
Correlation of absolute regional cerebral blood flow (rCBF) and percent change in rCBF between LDF and [14C]iodoantipyrine measurementssurrogate
Laser-Doppler flowmetry accurately measures relative changes in regional cerebral blood flow during focal cerebral ischemia in rats, but not absolute blood flow values.
Cite This Study
Dirnagl et al. (1989) studied this question.