February 1, 1984Journal of Biological ChemistryOpen Access

Mammalian skeletal muscle myosin light chain kinases. A comparison by antiserum cross-reactivity.

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Population

Skeletal muscle extracts of several mammalian species and different fibers

Comparison

Antiserum to rabbit skeletal muscle myosin light… vs Rabbit smooth muscle myosin light chain kinase

Design

Preclinical

Authors

MNMary H. NunnallyJohns Hopkins University JSJames T. StullHeart Failure / Cardiomyopathy

Discussion

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Implication

Distinguishes skeletal from smooth muscle MLCK isoforms; leaves open selective inhibitor development for cardiovascular research.

Structured PICO

Population

Skeletal muscle extracts of several mammalian species (mouse, guinea pig, rat, rabbit, dog, steer, cat, and chicken) and different fibers (slow twitch oxidative, fast twitch oxidative glycolytic, and fast twitch glycolytic)

Intervention

Antiserum to rabbit skeletal muscle myosin light chain kinase and Western blot analysis

Comparator

Rabbit smooth muscle myosin light chain kinase

Outcome

Subunit molecular weight and inhibition of catalytic activitysurrogate

Skeletal and smooth muscle myosin light chain kinases represent at least two distinct classes of enzymes, as demonstrated by differences in molecular weight and antiserum cross-reactivity.

Cite This Study

Nunnally et al. (1984) studied this question.

synapsesocial.com/papers/6a208eecb15f64b498301f5a https://doi.org/10.1016/s0021-9258(17)43475-2

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Bookmark

Mammalian skeletal muscle myosin light chain kinases. A comparison by antiserum cross-reactivity.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Mammalian skeletal muscle myosin light chain kinases. A comparison by antiserum cross-reactivity.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider