What question did this study set out to answer?

This research aims to purify and characterize a specific calcium-dependent protein kinase found in heart tissue.

July 1, 1982Journal of Biological ChemistryOpen Access

Phospholipid-sensitive Ca2+-dependent protein kinase from heart. I. Purification and general properties.

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This research aims to purify and characterize a specific calcium-dependent protein kinase found in heart tissue.
Utilized purification techniques including SDS-PAGE.
Analyzed enzyme activity in the presence of different phospholipid concentrations.
Employed buffer components like Pipes, Mes, and EGTA for optimal conditions.
Successfully purified the protein kinase with marked increase in activity upon calcium exposure.
Characterized the enzyme’s stability and response to different phospholipids.
Identified optimal buffer conditions that enhance enzyme performance.

Implication

Investigation reveals key properties of purified calcium-dependent protein kinase from heart tissue, indicating important biochemical roles.

Abstract

Pipes, 1,4-piperazinediethanesulfonic acid; Mes, 2-(N-morpholino)ethanesulfonic acid; SDS, sodium dode-cy1 sulfate; EGTA, ethylene glycol bis(p-aminoethyl ether)-N,N,N',N'-tetraacetic acid

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Cite This Study

Wise et al. (1982) studied this question.

synapsesocial.com/papers/6a208eecb15f64b498301f65 https://doi.org/10.1016/s0021-9258(18)34357-6

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Implication

Investigation reveals key properties of purified calcium-dependent protein kinase from heart tissue, indicating important biochemical roles.

Abstract

Pipes, 1,4-piperazinediethanesulfonic acid; Mes, 2-(N-morpholino)ethanesulfonic acid; SDS, sodium dode-cy1 sulfate; EGTA, ethylene glycol bis(p-aminoethyl ether)-N,N,N',N'-tetraacetic acid

Phospholipid-sensitive Ca2+-dependent protein kinase from heart. I. Purification and general properties.

Key Points

Implication

Abstract

Discussion

Cite This Study

Phospholipid-sensitive Ca2+-dependent protein kinase from heart. I. Purification and general properties.

Key Points

Implication

Abstract

Discussion

Cite This Study

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