The effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on intracellular Ca2+ handling in rat ventricular myocytes
Key result
In rat ventricular myocytes, 10 µM TBQ decreased the amplitude of the systolic Ca transient by 48%, the rate of decay by 54%, and the peak L-type Ca current by 23%, indicating it is not a specific SERCA inhibitor.
Structured PICO
P
Population
In vitro study using ventricular myocytes isolated from male Wistar rats to assess the effects of TBQ on intracellular calcium handling.
I
Intervention
2,5-Di-(tert-butyl)-1,4-benzohydroquinone (TBQ) (10μM and 100μM)
O
Outcome
Intracellular Ca2+ handling parameters (systolic Ca decay rate, systolic Ca transient amplitude, SR Ca content, L-type Ca current)surrogate
Main Result
p-value: p=<0.05
TBQ is not a specific inhibitor of SERCA in rat ventricular myocytes, as it also affects L-type Ca currents and activates an ATP-dependent potassium current.
Limitations
- In vitro study on rat myocytes, which may not fully translate to human cardiac physiology
- TBQ has off-target effects on L-type Ca current and activates an outward potassium current
Authors
Discussion
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Implication
TBQ lacks SERCA specificity in rat myocytes; leaves open need for selective inhibitors in calcium research.
Cite This Study
Miller et al. (2015) studied Healthy rat ventricular myocytes (in vitro). 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) vs. Baseline (control conditions prior to TBQ application) was evaluated on Amplitude of the systolic Ca transient and rate of decay (p=<0.05). In rat ventricular myocytes, 10 µM TBQ decreased the amplitude of the systolic Ca transient by 48%, the rate of decay by 54%, and the peak L-type Ca current by 23%, indicating it is not a specific SERCA inhibitor.