Identification of genes encoding potentially amyloidogenic proteins that take part in the regulation of nonsense suppression in yeast Saccharomyces cerevisiae

Previously, we demonstrated that SUP35 N-terminal deletion creates a specific genetic back-ground permitting the identification of novel genes and epigenetic determinants controlling nonsense suppression. In the present study, using a genomic screen, we found three genes encoding potentially amyloidogenic proteins, whose overexpression affects nonsense suppression in the strain producing chimeric Aβ-Sup35MC protein on the background of SUP35 deletion encoding releasing factor eRF3. These genes, NAB2, NAB3, and VTS1, were found to participate in the regulation of nonsense suppression in yeast S. cerevisiae.