What question did this study set out to answer?

To analyze the levels and distribution of 5-methylcytosine in human DNA across different tissues and cell types.

January 1, 1982Nucleic Acids ResearchOpen Access

Amount and distribution of 5-methylcytosine in human DNA from different types of tissues or cells

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Analysis reveals significant differences in DNA methylation across various human tissues and cells, indicating potential biological relevance.

To analyze the levels and distribution of 5-methylcytosine in human DNA across different tissues and cell types.
Analyzed DNA from seven different human tissues and eight homogeneous cell populations.
Measured 5-methylcytosine content in mole percent using specific biochemical techniques.
Compared methylation levels and distributions across tissue types and cell lines.
Brain and thymus tissues exhibited the highest 5-methylcytosine levels at 1.00 and 0.98 mole percent respectively.
Placental and sperm DNA showed the lowest methylation levels at 0.76 and 0.84 mole percent, with significant differences (p < 0.01) from the former group.
Nonrepetitive DNA sequences were generally hypomethylated relative to unfractionated DNA, with little variation in distribution by tissue or cell type.

Ehrlich et al. (1982) studied this question.

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Analysis reveals significant differences in DNA methylation across various human tissues and cells, indicating potential biological relevance.