Key result
Single or double knockout of ID1 and ID3 genes in BHK-21 cells promoted the replication of Foot-and-mouth disease virus and resulted in significant host gene expression changes.
Why the study?
Foot-and-mouth disease virus causes serious livestock losses, and the role of inhibitor of DNA binding proteins in viral infection is rarely known.
Knockout of ID1 and ID3 genes in BHK-21 cells promotes FMDV replication and significantly alters host gene expression, providing a basis for understanding host-virus interactions.
Foot-and-mouth disease virus (FMDV) is an important pathogen that harms cloven-hoofed animals and has caused serious losses to livestock production since its discovery. Furthermore, inhibitor of DNA binding (ID) proteins have been thoroughly studied in tumorigenesis, differentiation and metastasis, but its role in viral infection is rarely known. In this study, three gene knockout cell lines ID1 KO, ID3 KO, ID1/3 KO were obtained based on BHK-21 cells. We found that ID1 and ID3 genes single or double knockout promote the replication of FMDV. Moreover, compared with negative control cells during virus infection, there were 551 up-regulated genes and 1222 down-regulated genes in the ID1 KO cell line; 916 up-regulated genes and 1845 down-regulated genes in the ID3 KO cell line; 810 up-regulated genes and 1566 down-regulated genes in ID1/3 KO cell line. Further genes expression patterns verification results also showed a good correlation between the data of RT-qRCR and RNA-seq. These findings provide a basis for studying the relevant mechanisms between host genes and ID genes during FMDV infection.
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Luo et al. (2021) studied Foot-and-mouth disease virus (FMDV) infection. ID1 and ID3 gene knockout vs. Negative control cells was evaluated on FMDV replication and gene expression changes. Single or double knockout of ID1 and ID3 genes in BHK-21 cells promoted the replication of Foot-and-mouth disease virus and resulted in significant host gene expression changes.
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