Key result
Expression of antisense RNA against NTPDase6 in bovine kidney cells restricted foot-and-mouth disease virus propagation, reducing virus yield by up to 98-99% at a low multiplicity of infection.
Population
Bovine kidney cell line (LF-BK) highly susceptible to foot-and-mouth disease virus (FMDV) infection
Comparison
Antisense RNA expression of human expressed… vs Naïve/wild-type LF-BK tTA cells
Design
Preclinical
Authors
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NTPDase6 antisense RNA potently inhibits FMDV in bovine cells; leaves open translation to in vivo livestock therapeutics.
Inactivation of the host cellular gene NTPDase6 via antisense RNA restricts foot-and-mouth disease virus propagation by impairing viral RNA and protein synthesis.
Piccone et al. (2009) studied Foot-and-mouth disease virus (FMDV) infection. Antisense RNA inactivation of NTPDase6 vs. Wild-type LF-BK tTA cells was evaluated on Viral propagation and infectivity. Expression of antisense RNA against NTPDase6 in bovine kidney cells restricted foot-and-mouth disease virus propagation, reducing virus yield by up to 98-99% at a low multiplicity of infection.
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