Key result
Overexpression of caveolin-1 in HEK-293 cells at levels 10 to 40 times that of parental cells did not significantly alter insulin receptor expression or ligand-dependent signaling.
Population
HEK-293 cells (human embryonic kidney cells) with low endogenous caveolin-1
Comparison
Expression of caveolin-1 at various levels vs Parental HEK-293 cells with low endogenous…
Design
Preclinical
Authors
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Insulin receptor function appears independent of caveolin-1 in HEK-293 cells; leaves open its role in primary tissues or disease models.
Insulin receptor expression and ligand-dependent signaling are independent of caveolin-1 expression levels in HEK-293 cells.
Wharton et al. (2005) studied this question. Caveolin-1 overexpression vs. Parental HEK-293 cells was evaluated on Insulin receptor amount, insulin-dependent receptor autophosphorylation, and IRS1 tyrosine phosphorylation. Overexpression of caveolin-1 in HEK-293 cells at levels 10 to 40 times that of parental cells did not significantly alter insulin receptor expression or ligand-dependent signaling.
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