What is the clinical evidence from this study?

Study design: Other. Intervention: Long multiple fusion (long PCR-based fusion). Primary outcome: Assembly of linear DNA constructs up to 20 kb.

January 21, 2004Nucleic Acids ResearchOpen Access

Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously

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Key result

Long multiple fusion using a Pfu polymerase mix successfully assembled linear DNA constructs up to 20 kb with an accuracy of at least one error per 6.6 kb.

Population

DNA fragments for assembly

Design

Preclinical

Authors

NSNikolai A ShevchukChildren's National

Discussion

Member takes

Implication

Supports advanced DNA assembly in preclinical models; leaves open clinical translation.

Structured PICO

Population

DNA fragments for assembly

Intervention

Long multiple fusion method using Pfu polymerase mix

Outcome

Successful assembly of linear DNA constructs up to 20 kb

The long multiple fusion method allows for precise, simultaneous assembly of multiple DNA fragments up to 20 kb, facilitating complex genetic engineering projects.

Cite This Study

Nikolai A Shevchuk (2004) studied this question. Long multiple fusion (long PCR-based fusion) was evaluated on Assembly of linear DNA constructs up to 20 kb. Long multiple fusion using a Pfu polymerase mix successfully assembled linear DNA constructs up to 20 kb with an accuracy of at least one error per 6.6 kb.

synapsesocial.com/papers/6a20e4465496711a5f2aa7cd https://doi.org/10.1093/nar/gnh014

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Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider