What question did this study set out to answer?

This research aims to identify the epitopes on the SARS-CoV spike protein that induce neutralizing antibody responses.

April 1, 2005The Journal of Immunology

Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein Contains Multiple Conformation-Dependent Epitopes that Induce Highly Potent Neutralizing Antibodies

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Authors

YHYuxian HeSun Yat-sen University HLHong LüBoston University PSPamela SiddiquiNew York Blood Center

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Implication

Randomized trial demonstrates high titer neutralizing antibody responses in mice using SARS-CoV receptor-binding domain, suggesting potential for vaccine development.

Key Points

This research aims to identify the epitopes on the SARS-CoV spike protein that induce neutralizing antibody responses.
Characterization of receptor-binding domain from SARS-CoV spike protein residues 318-510.
Fusion protein RBD linked to human IgG1 Fc fragment was used to immunize mice.
Antibody responses were analyzed using ELISA and binding competition assays.
Mice produced high titer RBD-specific antibodies with neutralization titers of 1/15,360 for SARS-CoV and 1/24,737 for SARS pseudovirus.
Identified six groups of conformation-dependent epitopes and two linear epitopes on the RBD.
23 of 25 mAbs targeting conformational epitopes demonstrated potent neutralizing activity with doses ranging from 0.005 to 6.569 microg/ml.

Cite This Study

He et al. (2005) studied this question.

synapsesocial.com/papers/6a20eb54f76165bc1beccaa6 https://doi.org/10.4049/jimmunol.174.8.4908

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Implication

Randomized trial demonstrates high titer neutralizing antibody responses in mice using SARS-CoV receptor-binding domain, suggesting potential for vaccine development.

Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein Contains Multiple Conformation-Dependent Epitopes that Induce Highly Potent Neutralizing Antibodies

Discussion

Member takes

Implication

Key Points

Cite This Study

Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein Contains Multiple Conformation-Dependent Epitopes that Induce Highly Potent Neutralizing Antibodies

Discussion

Member takes

Implication

Key Points

Cite This Study

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