What question did this study set out to answer?

To evaluate the diagnostic utility and specificity of the SARS-CoV-2 spike protein receptor-binding domain (RBD) as an antigen for serological surveillance.

June 11, 2020Science ImmunologyOpen Access

The receptor-binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients

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Authors

LPLakshmanane PremkumarUniversity of North Carolina at Charlotte BSBruno Segovia-ChumbezUniversity of North Carolina at Chapel Hill RJRamesh JadiUniversity of North Carolina at Chapel Hill

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Implication

Serological study demonstrates high diagnostic accuracy of spike receptor-binding domain antibodies in COVID-19 patients, indicating their utility as correlates of viral neutralization.

Key Points

To evaluate the diagnostic utility and specificity of the SARS-CoV-2 spike protein receptor-binding domain (RBD) as an antigen for serological surveillance.
Tested a panel of human sera from 63 SARS-CoV-2 patients and 71 negative control subjects.
Assessed cross-reactivity using hyperimmune animal sera raised against other zoonotic coronaviruses to evaluate recombinant SARS-CoV-2 RBD antigen performance.
By day 9 after symptom onset, recombinant SARS-CoV-2 RBD antigen exhibited 98% sensitivity and 100% specificity for SARS-CoV-induced antibodies.
Levels of RBD-binding antibodies strongly correlated with SARS-CoV-2 neutralizing antibody titers in patient sera.

Cite This Study

Premkumar et al. (2020) studied this question.

synapsesocial.com/papers/6a6f577bce524a4339c34eec https://doi.org/10.1126/sciimmunol.abc8413

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Implication

Serological study demonstrates high diagnostic accuracy of spike receptor-binding domain antibodies in COVID-19 patients, indicating their utility as correlates of viral neutralization.

The receptor-binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients

Discussion

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Implication

Key Points

Cite This Study

The receptor-binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients

Discussion

Member takes

Implication

Key Points

Cite This Study

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