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This investigation aims to analyze glycoproteins in different rat cell surface membranes and identify their subunit patterns.

Glycoproteins of Cell Surfaces

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This investigation aims to analyze glycoproteins in different rat cell surface membranes and identify their subunit patterns.
Utilized acrylamide gel electrophoresis with discontinuous buffers and SDS.
Membranes from liver, kidney brush border, and erythrocyte ghosts were solubilized and analyzed.
Subunits were stained and their sizes determined using molecular weight markers.
Each cell membrane exhibited a distinct glycoprotein subunit pattern with 6 to 11 different sizes.
Liver and kidney brush border share four identically sized subunits (250,000, 195,000, 130,000, and 96,000 Da).
All prominent Schiff-positive glycoproteins were found to contain sialic acid.

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Randomized trial investigates glycoprotein patterns in rat cell membranes, revealing unique subunit compositions.

Glossmann et al. (1971) studied this question.

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