What question did this study set out to answer?

To determine whether a carbohydrate-rich coat exists on the outer surface of mammalian cells across diverse tissue types.

January 1, 1967The Journal of Cell BiologyOpen Access

Electron Microscope Observations on the Carbohydrate-Rich Cell Coat Present at the Surface of Cells in the Rat

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Authors

ARA. RambourgCentre National de la Recherche Scientifique CLC. P. LeblondAmerican Museum of Natural History

Discussion

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Implication

Histochemical electron microscopy reveals a continuous carbohydrate-rich cell coat across diverse rat tissues, suggesting essential roles in cellular adhesion and environmental interactions.

Key Points

To determine whether a carbohydrate-rich coat exists on the outer surface of mammalian cells across diverse tissue types.
Stained multiple rat tissues with periodic acid-silver methenamine to detect glycoproteins by electron microscopy.
Applied colloidal thorium staining to identify acidic carbohydrates on the outer cell surface.
Nearly all examined cell types exhibited a thin external coat of stained material, with increased density at microvilli, lateral interspaces, and synaptic clefts.
The stained layer was continuous through desmosomes and terminal bars but vanished where plasma membranes fused to form tight junctions.
Staining with periodic acid-silver methenamine and colloidal thorium confirmed that the cell coat contains both glycoproteins and acidic carbohydrate residues.

Cite This Study

Rambourg et al. (1967) studied this question.

synapsesocial.com/papers/6a717136fe4101aa97e09377 https://doi.org/10.1083/jcb.32.1.27

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January 1, 1967The Journal of Cell BiologyOpen Access

Electron Microscope Observations on the Carbohydrate-Rich Cell Coat Present at the Surface of Cells in the Rat

View Full Paper

Ask AI

Bookmark

Authors

ARA. RambourgCentre National de la Recherche Scientifique CLC. P. LeblondAmerican Museum of Natural History

Discussion

Member takes

Implication

Histochemical electron microscopy reveals a continuous carbohydrate-rich cell coat across diverse rat tissues, suggesting essential roles in cellular adhesion and environmental interactions.

Key Points

To determine whether a carbohydrate-rich coat exists on the outer surface of mammalian cells across diverse tissue types.
Stained multiple rat tissues with periodic acid-silver methenamine to detect glycoproteins by electron microscopy.
Applied colloidal thorium staining to identify acidic carbohydrates on the outer cell surface.
Nearly all examined cell types exhibited a thin external coat of stained material, with increased density at microvilli, lateral interspaces, and synaptic clefts.
The stained layer was continuous through desmosomes and terminal bars but vanished where plasma membranes fused to form tight junctions.
Staining with periodic acid-silver methenamine and colloidal thorium confirmed that the cell coat contains both glycoproteins and acidic carbohydrate residues.

Cite This Study

Rambourg et al. (1967) studied this question.

synapsesocial.com/papers/6a717136fe4101aa97e09377 https://doi.org/10.1083/jcb.32.1.27

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