Key result
Dual glycogen-protein staining reveals two distinct glycosome classes in cardiac tissue.
Why the study?
The structural demonstration of glycosomes as protein-glycogen complexes in cardiac conduction fibers had not been simultaneously achieved for glycogen and protein.
Population
Cardiac conduction fibers tissue samples
Comparison
Sections stained with PA-TSC-SP for glycogen vs control sections with H2O2; tissue fixed with glutaraldehyde and OsO4 with or without uranyl acetate treatment
Design
Histochemical staining study with different fixation and staining protocols
Authors
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Reveals glycosome structure in conduction fibers; extends basic histochemistry but leaves functional and clinical roles open.
Krystyna Rybicka (1981) studied Cardiac tissue. En bloc treatment with uranyl acetate and PA-TSC-SP staining vs. Glutaraldehyde and OsO4 fixation alone / H2O2 control was evaluated on Demonstration of glycogen and protein in glycosomes. Simultaneous staining of glycogen and protein in cardiac tissue treated en bloc with uranyl acetate revealed two classes of glycosomes, demonstrating the structure of intact glycosomes.
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