Key result
Neolignans isolated from Magnolia obovata fruits significantly inhibited nitric oxide production in LPS-induced RAW 264.7 cells, with IC50 values ranging from 3.3 to 15.8 µM.
Neolignans isolated from Magnolia obovata demonstrate significant inhibitory activity against NO production in LPS-induced RAW 264.7 cells.
Hypothesis-generating for anti-inflammatory effects; leaves open clinical translation pending in vivo studies.
Three new neolignans, named 9-methoxyobovatol (6), magnobovatol (7), and 2-hydroxyobovaaldehyde (9), along with six known ones, magnolol (1), honokiol (2), isomagnolol (3), obovatol (4), obovatal (5), and obovaaldehyde (8), were isolated from the fruits of Magnolia obovata using silica gel and ODS column chromatography. From the results of spectroscopic data including EIMS, IR, ¹H- and ¹³C-NMR, DEPT, and 2D-NMR (gCOSY, gHSQC, gHMBC), the chemical structures were determined. All isolated compounds were evaluated for inhibition activity on nitric oxide production in LPS-induced RAW 264.7 cells, and compounds 1–4, 6, 7, and 9 showed significant activity with IC₅₀ values of 15.8 ± 0.3, 3.3 ± 1.2, 14.1 ± 0.9, 6.2 ± 1.2, 14.8 ± 2.3, 14.2 ± 1.2, and 14.8 ± 3.2 µM, respectively, without any visible toxic effect.
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Seo et al. (2013) studied Nitric oxide production in LPS-induced RAW 264.7 cells. Neolignans from Magnolia obovata was evaluated on Inhibition of nitric oxide production. Neolignans isolated from Magnolia obovata fruits significantly inhibited nitric oxide production in LPS-induced RAW 264.7 cells, with IC50 values ranging from 3.3 to 15.8 µM.
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