Comparison of the survival of endothelium‐derived relaxing factor and nitric oxide within the isolated perfused mesenteric arterial bed of the rat
Key result
Exogenously applied nitric oxide survived passage through the rat mesenteric vascular bed to relax rabbit aortic strips, whereas endothelium-derived relaxing factor released by acetylcholine did not.
Population
rat isolated perfused mesenteric vascular bed and rabbit aortic strips (RbAs)
Comparison
Acetylcholine to release endothelium-derived… vs Exogenously applied nitric oxide
Design
Preclinical
Authors
Discussion
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Implication
Questions EDRF-NO equivalence in intact vascular beds; leaves open abluminal release effects in vivo.
Structured PICO
P
Population
rat isolated perfused mesenteric vascular bed and rabbit aortic strips (RbAs)
I
Intervention
Acetylcholine (ACh) (1.7 pmol-300 nmol) to release endothelium-derived relaxing factor (EDRF)
C
Comparator
Exogenously applied nitric oxide (NO) (0.1-10 nmol)
O
Outcome
Survival of EDRF vs NO in the effluent measured by direct bioassay on rabbit aortic stripssurrogate
Native EDRF released by ACh does not survive passage through the rat mesenteric vascular bed, unlike exogenous NO, possibly due to differences in abluminal versus luminal release volumes.
Cite This Study
Warner et al. (1989) studied this question. Acetylcholine (ACh) releasing EDRF vs. Nitric oxide (NO) was evaluated on Survival of EDRF vs NO in the effluent (bioassayable relaxation of rabbit aortic strips). Exogenously applied nitric oxide survived passage through the rat mesenteric vascular bed to relax rabbit aortic strips, whereas endothelium-derived relaxing factor released by acetylcholine did not.