Anatomical location, sex, and age influence murine arterial circumferential cyclic strain before and during dobutamine infusion

Figures

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  Figure 1. Representative data from the four anatomical locations investigated. A: Coronal maximum intensity projection from a multiple thin slab 3D acquisition. B: Single cross‐sectional CINE frame from each anatomical location studied (shown on the same scale). C: Twelve CINE frames from the infrarenal aorta of a young male mouse. Dotted circles represent the perimeter of the vessel at diastole and are shown in each image to visualize vessel expansion across the cardiac cycle. Solid arrows are shown for end‐diastole and the dotted arrow represents peak‐systole.

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  Figure 2. Green‐Lagrange circumferential cyclic strain of the infrarenal aorta across the cardiac cycle for male (A,B) and female (C,D) mice, before (left) and during (right) dobutamine infusion. Young animals are shown in gray (n = 5 male, n = 5 female) and mature animals are shown in black (n = 5 male, n = 5 female). Dobutamine infusion increased cyclic strain.

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  Figure 3. Maximum Green‐Lagrange circumferential cyclic strain at baseline and during the infusion of dobutamine. Baseline and dobutamine maximum cyclic strain are displayed side‐by‐side for each group at each of the four locations (n = 5 per group). Significant differences based on location (#) or dobutamine (*) within a group; and age (Δ) or sex (†) at a given location. Subscripts correspond to baseline (b) or dobutamine (d).

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  Figure 4. Change in maximum cyclic strain at the carotid, suprarenal, infrarenal, and iliac locations for each group. Significant differences based on age (Δ) or sex (†).

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  Figure 5. Cross‐sectional area of the infrarenal aorta across the cardiac cycle, for male (A,B) and female (C,D) mice, before (left) and during (right) dobutamine infusion. Young animals are shown in gray (n = 5 male, n = 5 female) and mature animals are shown in black (n = 5 male, n = 5 female). Dobutamine infusion decreased area.

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  Figure 6. Average cross‐sectional area at baseline and during the infusion of dobutamine. Baseline and dobutamine average area are displayed side‐by‐side for each group at each of the four locations (n = 5 per group). Significant differences based on dobutamine (*) within a group; and age (Δ) or sex (†) at a given location. Subscripts correspond to baseline (b) or dobutamine (d).

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  Figure 7. Relative change in average area at the carotid, suprarenal, infrarenal, and iliac locations for each group. Significant differences based on age (Δ) or sex (†).

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  Figure 8. Comparison of change in maximum strain and relative change in average area due to the administration of dobutamine, for young males (A), mature males (B), young females (C), and mature females (D); (n = 5 per group). Typically, a larger relative reduction in area was matched with a larger increase in maximum strain.