September 1, 1993The Journal of Infectious Diseases
Human Peripheral Blood Xenografts in the SCID Mouse: Characterization of Immunologic Reconstitution
Key result
Adoptive transfer of human peripheral blood mononuclear cells into SCID mice resulted in successful immune reconstitution in over 90% of cases, as evidenced by human immunoglobulin production.
Population
1800 C.B-17 scid-/scid- mice
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
May advance preclinical immunologic research in humanized mice; leaves open human translation pending validation.
Structured PICO
P
Population
1,800 C.B-17 scid-/scid-mice receiving adoptive transfer of human peripheral blood mononuclear cells to characterize immune reconstitution.
I
Intervention
Adoptive transfer of human peripheral blood mononuclear cells
O
Outcome
Characterization of immune reconstitutions (human immunoglobulin production, cell recovery, soluble CD8 and IL-2 receptors, proliferation response)surrogate
The hu-PBL-SCID mouse model shows promise for immunologic studies, with over 90% successful immune reconstitution, though inherent variation must be minimized.
Limitations
- Inherent variation in the system depending on donor, cell number, and cell type
- Inherent variation in the system with donor, cell number, and cell type
Cite This Study
Hesselton et al. (1993) studied Immune reconstitution model (n=1,800). Adoptive transfer of human peripheral blood mononuclear cells was evaluated on Successful immune reconstitution (evidenced by human immunoglobulin production). Adoptive transfer of human peripheral blood mononuclear cells into SCID mice resulted in successful immune reconstitution in over 90% of cases, as evidenced by human immunoglobulin production.