What is the clinical evidence from this study?

Study design: Other. Population: Nitroglycerin tolerance. Intervention: Nitroglycerin (GTN) tolerance induction vs. Control vessels. Primary outcome: Stimulatory action of nitroprusside (NP) or GTN/cysteine on guanylate cyclase (GC) (50-60% reduction).

What question did this study set out to answer?

This research aims to investigate the effects of nitroglycerin tolerance on cGMP turnover in bovine mesenteric arteries.

September 1, 1984Acta Pharmacologica et Toxicologica

Nitroglycerin Tolerancein Vitro: Effect on cGMP Turnover in Vascular Smooth Muscle

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Key result

Nitroglycerin tolerance in bovine mesenteric arteries reduced the stimulatory action of nitroprusside or GTN/cysteine on guanylate cyclase by 50-60% compared to control vessels.

Why the study?

Does nitroglycerin tolerance affect cGMP turnover and guanylate cyclase activity in bovine mesenteric arteries?

Population

Bovine mesenteric arteries (BMA)

Comparison

Incubation with high concentrations of… vs Control vessels

Design

Preclinical

Authors

KAKrister L. AxelssonCalifornia State University, East Bay JKJ. KarlssonLinköping University

Discussion

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Implication

Hypothesis-generating for guanylate cyclase role in nitrate tolerance; leaves open clinical translation.

Key Points

This research aims to investigate the effects of nitroglycerin tolerance on cGMP turnover in bovine mesenteric arteries.
Bovine mesenteric arteries incubated with high concentrations of nitroglycerin at elevated pH to induce tolerance.
Assessed the effects of nitroprusside and nitroglycerin/cysteine on guanylate cyclase activity.
Evaluated nitrite formation and the role of thiols in restoring guanylate cyclase activity.
Guanylate cyclase activity in nitroglycerin-tolerant vessels was reduced by 50-60% compared to control vessels.
Tolerance induction did not change the rate of nitrite formation from nitroglycerin.
Exogenous thiols failed to restore guanylate cyclase activity to control levels, indicating a direct impact of tolerance on the enzyme.

Structured PICO

Does nitroglycerin tolerance affect cGMP turnover and guanylate cyclase activity in bovine mesenteric arteries?

Population

Bovine mesenteric arteries (BMA)

Intervention

Incubation with high concentrations of nitroglycerin (GTN) at elevated pH to induce tolerance

Comparator

Control vessels

Outcome

Guanylate cyclase (GC) activity and cGMP turnoversurrogate

Main Result

Effect estimate: 50-60% reduction

Nitroglycerin tolerance in vascular smooth muscle appears to involve a direct reduction in guanylate cyclase activity rather than changes in nitrite formation or cGMP-phosphodiesterase activity.

Cite This Study

Axelsson et al. (1984) studied Nitroglycerin tolerance. Nitroglycerin (GTN) tolerance induction vs. Control vessels was evaluated on Stimulatory action of nitroprusside (NP) or GTN/cysteine on guanylate cyclase (GC) (50-60% reduction). Nitroglycerin tolerance in bovine mesenteric arteries reduced the stimulatory action of nitroprusside or GTN/cysteine on guanylate cyclase by 50-60% compared to control vessels.

synapsesocial.com/papers/6a21e4e1de966c0841b92a43 https://doi.org/10.1111/j.1600-0773.1984.tb02038.x

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Nitroglycerin Tolerancein Vitro: Effect on cGMP Turnover in Vascular Smooth Muscle

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Nitroglycerin Tolerancein Vitro: Effect on cGMP Turnover in Vascular Smooth Muscle

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Also Consider

Also Consider