Novel Agonists of Adenosine Receptors in Animal Model of Acute Myocardial Infarction

Figures

• 

  Figure 1. Chemical structure of LASSBio-1027 and LASSBio-1860.

• 

  Figure 2. LASSBio-1027 and LASSBio-1860 on left ventricule (LV) wall thickness and internal diameter obtained through echocardiography in MI-induced animals. (A) Representative image of LV in M-Mode of Sham-vehicle and MI-induced. (B) Measurement of anterior wall thickness in systole; (C) Measurement of anterior wall thickness in diastole from animals treated with either vehicle or LASSBio-1027 or LASSBio-1860. *p<0.05 compared to Sham-vehicle. #p<0.05 compared to MI-vehicle. Data expressed as mean ± SEM. n = 7 animals/group.

• 

  Figure 3. LASSBio-1027 and LASSBio-1860 on left ventricule (LV) hemodynamics in MI-induced animals. (A) LV filling pressure (E/e’) after treatement whit vehicle or LASSBio-1027. (B) Ejection fraction after treatement whit vehicle or LASSBio-1027. (C) LV filling pressure (E/e’) after treatement whit vehicle or LASSBio-1860. (D) Ejection fraction after treatement whit vehicle or LASSBio-1860. *p<0.05 compared to Sham-vehicle. #p<0.05 compared to MI-vehicle. n = 7 animals/group.

• 

  Figure 4. LASSBio-1027 and LASSBio-1860 on left ventricule (LV) hemodynamics in MI-induced animals. LV end diastolic pressure from animals treated with either vehicle or LASSBio-1027 or LASSBio-1860. *p<0.05 compared to Sham-vehicle. #p<0.05 compared to MI-vehicle. n = 7 animals/group.

• 

  Figure 5. LASSBio-1027 and LASSBio-1860 on left ventricule (LV) histology in MI-induced animals. (A) Representative micrographs of LV sections with hematoxylin-eosin stain. (B) Interstitial cell density in MI border zone in LV tissue from animals treated with either vehicle or LASSBio-1027 or LASSBio-1860. *p<0.05 compared to MI-vehicle. #p<0.05 compared to Sham-vehicle. Data are expressed as mean ± SEM. n = 7 animals/group.

• 

  Figure 6. LASSBio-1027 and LASSBio-1860 on left ventricule (LV) histology in MI-induced animals. (A) Representative micrographs of LV sections with picro sirius red stain. (B) Collagen deposition in MI border zone in LV tissue from animals treated with either vehicle or LASSBio-1027 or LASSBio-1860. *p<0.05 compared to Sham-vehicle. #p<0.05 compared to MI-vehicle. n = 7 animals/group.

• 

  Figure 7. LASSBio-1027 and LASSBio-1860 on left ventricule (LV) immunohistochemistry in MI-induced animals. (A) Representative micrographs of LV sections with immunohistochemistry for smooth muscle α-actin (α-SMA) stain in negative control, Sham-vehicle and MI treated with vehicle, LASSBio-1027 or LASSBio-1860. (B) Percentage of stain for α-SMA in MI border zone in LV tissue, from animals treated with either vehicle or LASSBio-1027 or LASSBio-1860. *p<0.05 compared to Sham-vehicle. #p<0.05 compared to MI-vehicle. n = 7 animals/group.

• 

  Figure 8. LASSBio-1027 and LASSBio-1860 on left ventricule (LV) immunohistochemistry in MI-induced animals. (A) Representative micrographs of LV sections with immunohistochemistry for TNF-α stain in negative control, Sham-vehicle and MI treated with vehicle, LASSBio-1027 or LASSBio-1860. (B) Percentage of stain for TNF-α in MI border zone in LV tissue from animals treated with either vehicle or LASSBio-1027 or LASSBio-1860. *p<0.05 compared to Sham-vehicle. #p<0.05 compared to MI-vehicle. n = 7 animals/group.

• 

  Figure 9. LASSBio-1027 and LASSBio-1860 on left ventricule (LV) immunohistochemistry in MI-induced animals. (A) Representative micrographs of LV sections with immunohistochemistry for inducible nitric oxide synthase (iNOS) stain in negative control, Sham-vehicle and MI treated with vehicle, LASSBio-1027 or LASSBio-1860. (B) Percentage of stain for TNF-α in MI border zone in LV tissue from animals treated with either vehicle or LASSBio-1027 or LASSBio-1860. *p<0.05 compared to Sham-vehicle. #p<0.05 compared to MI-vehicle. n = 7 animals/group.

• 

  Figure 10. LASSBio-1027 and LASSBio-1860 on left ventricule (LV) immunohistochemistry in MI-induced animals. (A) Representative micrographs of LV sections with immunohistochemistry for c-Fos stain, Sham-vehicle and MI treated with vehicle, LASSBio-1027 or LASSBio-1860; Arrows indicate nuclei stain by anti-c-Fos antibody. (B) Percentage of marker nuclei by anti-cFos in relation to the number of total nuclei in MI border zone in LV tissue from animals treated with either vehicle or LASSBio-1027 or LASSBio-1860. *p<0.05 compared to Sham-vehicle. #p<0.05 compared to MI-vehicle. n = 7 animals/group.