Key result
The presence of the leader ORF on the bicistronic 16S mRNA species reduces VP1 synthesis threefold relative to a similar RNA lacking it, largely via a leaky scanning mechanism.
Population
Monkey cells transfected with simian virus 40 (SV40) viral mutants
Design
Preclinical
Authors
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SV40 leader ORFs modulate virion protein ratios via leaky scanning; leaves open conservation and in vivo impact across polyomaviruses.
Leader-encoded open reading frames in SV40 modulate the absolute and relative rates of synthesis of virion proteins via leaky scanning mechanisms.
Sedman et al. (1989) studied Simian virus 40 (SV40) infection. Viral mutants with altered leader-encoded translation initiation signals or ORF lengths vs. Similar RNA lacking the leader ORF was evaluated on Levels of initiation at and leaky scanning past each initiation signal and synthesis of virion proteins. The presence of the leader ORF on the bicistronic 16S mRNA species reduces VP1 synthesis threefold relative to a similar RNA lacking it, largely via a leaky scanning mechanism.
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