What question did this study set out to answer?

This research aims to investigate how myofibrillar ATPase activity varies with fiber type and temperature in human skeletal muscle.

June 1, 1996The Journal of PhysiologyOpen Access

Myofibrillar ATPase activity in skinned human skeletal muscle fibres: fibre type and temperature dependence.

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Population

Chemically skinned skeletal muscle fibres from human rectus abdominis and vastus lateralis muscle

Design

Preclinical

Authors

GSGer J.M. StienenHeart Failure & Transplant JKJ.L. KiersFrieslandCampina (Netherlands)RBRoberto BottinelliHeart Failure / Cardiomyopathy

Discussion

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Implication

Fibre-type ATP consumption differences in animal skeletal muscle warrant caution in clinical extrapolation; leaves open translation to human cardiac energetics.

Key Points

This research aims to investigate how myofibrillar ATPase activity varies with fiber type and temperature in human skeletal muscle.
Analyzed myofibrillar ATP consumption and isometric tension in skinned skeletal muscle fibres from rectus abdominis and vastus lateralis.
Classified fibers into four groups based on myosin heavy chain composition: I, IIA, IIB, and mixed.
Measured ATP consumption at various temperatures (12 to 30 degrees C) and compared results to previous findings in rat fibers.
ATP consumption at 20 degrees C ranged from 0.41 mmol l-1 s-1 in type IIB fibers to 0.10 mmol l-1 s-1 in type I fibers.
The tension cost was lower in human fibers at 12 degrees C than previously found in rats.
The Q10 value (relative increase in ATPase activity per 10 degrees C change) was temperature independent at 2.60.

Structured PICO

Population

Chemically skinned skeletal muscle fibres from human rectus abdominis and vastus lateralis muscle

Intervention

Temperature variation (12 to 30 degrees C)

Outcome

Myofibrillar ATP consumption and isometric tension (P0)surrogate

The study demonstrates that myofibrillar ATP consumption and tension cost in human skeletal muscle vary significantly by fibre type and are temperature-dependent.

Cite This Study

Stienen et al. (1996) studied this question.

synapsesocial.com/papers/6a220e2ac7675eb28596f6f5 https://doi.org/10.1113/jphysiol.1996.sp021384

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Implication

Fibre-type ATP consumption differences in animal skeletal muscle warrant caution in clinical extrapolation; leaves open translation to human cardiac energetics.

Myofibrillar ATPase activity in skinned human skeletal muscle fibres: fibre type and temperature dependence.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Myofibrillar ATPase activity in skinned human skeletal muscle fibres: fibre type and temperature dependence.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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