Key result
Optical multisite monitoring of membrane potentials using di-4-ANEPPS during voltage clamp in single cardiomyocytes showed a 5% change in fluorescence intensity per 100mV with a linear relationship.
Simultaneous optical multisite monitoring of membrane potentials provides a calibrated method to interpret voltage clamp experiments with large and fast currents in single cardiomyocytes.
Does not inform clinical practice; leaves open calibrated optical monitoring for cardiomyocyte voltage studies.
We have performed voltage clamp experiments in single cardiomyocytes combined with simultaneous optical multisite monitoring of membrane potentials. The fluorescent potential sensitive dye di-4-ANEPPS was used for staining and an argon ion laser for excitation of fluorescence. The percentage change of the fluorescence light intensity was about 5% per lOOmV, and showed a linear relationship (within the limits of noise). Optical signals obtained simultaneously from different measuring spots on a cell sometimes showed timelags of about 100 ps. Normalizing the optical signals to the clamp steps allowed to obtain calibrated membrane potential values within a cell. This promises to be a powerful method to interpret voltage clamp experiments with large and fast currents.
No takes yet. Share an insight, caveat, or question.
Windisch et al. (2005) studied this question. Optical multisite monitoring of membrane potentials using di-4-ANEPPS during voltage clamp was evaluated on Percentage change of fluorescence light intensity and timelags. Optical multisite monitoring of membrane potentials using di-4-ANEPPS during voltage clamp in single cardiomyocytes showed a 5% change in fluorescence intensity per 100mV with a linear relationship.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: