Immunohistochemical insights into Saffold virus infection of the brain of juvenile AG129 mice

Figures

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  Fig. 1. Immunohistochemical co-localisation of SAFV VP1 with NeuN or GFAP. Brain sections of 6dpi SAFV infected 2 week-old AG129 mice were stain with anti-SAFV VP1 and anti-NeuN or anti-GFAP. Stained SAFV infected or uninfected mice brain sections were viewed and taken with a confocal fluorescence microscope. SAFV VP1 is labelled in red, NeuN or GFAP is labelled in green, and DAPI is labelled in blue. Arrows point at examples of colocalisation

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  Fig. 2. Immunohistochemical co-localisation of cleaved caspase 3 with NeuN or GFAP. Brain sections tissue of 6 dpi SAFV infected or uninfected 2 week-old AG129 mice were stained with anti-cleaved caspase 3 and anti-GFAP or anti-NeuN. Resulting stain was viewed and taken with widefield or confocal fluorescence microscope. Cleaved caspase 3 is labelled in red, NeuN or GFAP is labelled in green, and DAPI is labelled in blue. Arrows point at examples of colocalisation

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  Fig. 3. Fluorescence images of MBP stained uninfected and SAFV infected brain. Brain sections of 6dpi 2 week-old AG129 mice or 35dpi 3–4 week-old AG129 mice were stained with anti-MBP antibodies. No observable demyelination was detected in any sections