Key result
A poly(A) leader sequence in mRNA constructs promotes the efficient formation of initiation complexes at cognate AUG initiation codons in the absence of translation initiation factors eIF3 and eIF4F.
Population
Eukaryotic mRNA constructs (in vivo and cell-free translation systems)
Comparison
Poly(A) leader sequence at the 5' end of mRNA vs Standard cap-dependent translation mechanism
Design
Preclinical
Authors
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May inform mRNA construct design for cardiac therapies; leaves open clinical translation from animal data.
A 5'-proximal poly(A) sequence in eukaryotic mRNA enables an alternative, cap-independent and ATP-independent mechanism of translation initiation that bypasses essential initiation factors eIF3 and eIF4F.
Shirokikh et al. (2008) studied this question. Poly(A) leader sequence vs. Natural capped β-globin mRNA was evaluated on Formation of 48S ribosomal initiation complexes. A poly(A) leader sequence in mRNA constructs promotes the efficient formation of initiation complexes at cognate AUG initiation codons in the absence of translation initiation factors eIF3 and eIF4F.
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