What question did this study set out to answer?

This research investigates the sequence and expression mechanisms of the Berne virus polymerase and its evolutionary ties with coronaviruses.

August 1, 1990Nucleic Acids ResearchOpen Access

The carboxyl-terminal part of the putative Berne virus polymerase is expressed by ribosomal frameshifting and contains sequence motifs which indicate that toro- and coronaviruses are evolutionary related

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Population

Berne virus (BEV) genome (torovirus prototype)

Comparison

Sequence analysis of the 3' part of the… vs Coronaviruses

Design

Preclinical

Authors

ESEric J. SnijderLeiden University JBJohan A. den BoonUniversity of Wisconsin–Madison PBPeter J. BredenbeekLeiden University Medical Center

Discussion

Member takes

Implication

Supports shared frameshifting mechanisms across virus families; leaves open functional validation in mammalian models.

Key Points

This research investigates the sequence and expression mechanisms of the Berne virus polymerase and its evolutionary ties with coronaviruses.
Analyzed the 3' part of the Berne virus polymerase gene, revealing overlapping open reading frames.
Determined the complete sequence of ORF1b at 6873 nucleotides.
Identified conserved amino acid domains through sequence comparison with coronaviruses.
Identified expression of ORF1b through ribosomal frameshifting akin to coronaviruses.
Four conserved domains were noted, suggesting evolutionary relationships between toro- and coronaviruses.
Found similarities in the tertiary RNA structures directing frameshifting, reinforcing genetic ties between these virus families.

Structured PICO

Population

Berne virus (BEV) genome (torovirus prototype)

Intervention

Sequence analysis of the 3' part (8 kb) of the polymerase gene

Comparator

Coronaviruses

Outcome

Sequence homology and structural similarities (ribosomal frameshifting, pseudoknot structure, conserved domains)surrogate

Sequence analysis of the Berne virus polymerase gene reveals structural and sequence homologies with coronaviruses, indicating a close evolutionary relationship.

Cite This Study

Snijder et al. (1990) studied this question.

synapsesocial.com/papers/6a224c231b095894fc4eed7d https://doi.org/10.1093/nar/18.15.4535

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The carboxyl-terminal part of the putative Berne virus polymerase is expressed by ribosomal frameshifting and contains sequence motifs which indicate that toro- and coronaviruses are evolutionary related

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

The carboxyl-terminal part of the putative Berne virus polymerase is expressed by ribosomal frameshifting and contains sequence motifs which indicate that toro- and coronaviruses are evolutionary related

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider