July 1, 1989Journal of Biological ChemistryOpen Access

Functional Consequences of Glutamate, Aspartate, Glutamine, and Asparagine Mutations in the Stalk Sector of the Ca2+-ATPase of Sarcoplasmic Reticulum

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Population

COS-1 cells expressing mutant sarcoplasmic reticulum Ca2+-ATPases

Design

Preclinical

Authors

DCDavid M. ClarkeMonash Medical Centre KMKei MaruyamaSaitama Medical University TLTip W. LooUniversity of Toronto

Discussion

Member takes

Implication

Asn111/Asn114 mutations impair SERCA Ca2+ transport in vitro; hypothesis-generating for cardiac calcium handling, requires in vivo validation before any clinical consideration.

Structured PICO

Population

COS-1 cells expressing mutant sarcoplasmic reticulum Ca2+-ATPases

Intervention

Oligonucleotide-directed site-specific mutagenesis of glutamate, glutamine, aspartate, or asparagine residues within the stalk sector

Outcome

Ca2+ transport function and phosphoenzyme formationsurrogate

Acidic and amidated residues in the stalk region of the SR Ca2+-ATPase are not required for high-affinity Ca2+ binding but may help sequester and channel cytoplasmic Ca2+ for translocation.

Cite This Study

Clarke et al. (1989) studied this question.

synapsesocial.com/papers/6a224c433b8e99975a4ecb8a https://doi.org/10.1016/s0021-9258(18)60455-7

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Implication

Asn111/Asn114 mutations impair SERCA Ca2+ transport in vitro; hypothesis-generating for cardiac calcium handling, requires in vivo validation before any clinical consideration.

Functional Consequences of Glutamate, Aspartate, Glutamine, and Asparagine Mutations in the Stalk Sector of the Ca2+-ATPase of Sarcoplasmic Reticulum

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Functional Consequences of Glutamate, Aspartate, Glutamine, and Asparagine Mutations in the Stalk Sector of the Ca2+-ATPase of Sarcoplasmic Reticulum

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider