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February 1, 1987The Journal of Biochemistry

Reactive Sulfhydryl Groups of Sarcoplasmic Reticulum ATPase. II. Site of Labeling with Iodoacetamide and Its fluorescent Derivative1

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Key result

Iodoacetamide and its fluorescent derivative IAEDANS specifically bind to the identical site (Cys 674) on the C-terminal half of sarcoplasmic reticulum Ca2+,Mg2+-ATPase.

Population

Sarcoplasmic reticulum (SR) membranes

Design

Preclinical

Authors

TYTetsuro YamashitaIwate UniversityMKMasao KawakitaFlorida State University

Discussion

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Implication

Cys 674 labeling may enable SERCA studies in cardiac models; leaves open its functional or therapeutic relevance in humans.

Structured PICO

P
Population
Sarcoplasmic reticulum (SR) membranes
I
Intervention
Treatment with iodoacetamide (IAA) and its fluorescent derivative 5-(2-iodoacetamidoethyl) amino-naphthalene-1-sulfonate (IAEDANS)
O
Outcome
Identification of the specific binding site on the C-terminal half of sarcoplasmic reticulum Ca2+,Mg2+-ATPasesurrogate

The study identifies Cys 674 as the specific binding site for iodoacetamide and IAEDANS on the sarcoplasmic reticulum Ca2+,Mg2+-ATPase.

Cite This Study

Yamashita et al. (1987) studied this question. Iodoacetamide (IAA) and IAEDANS was evaluated on Identification of specific binding site on sarcoplasmic reticulum Ca2+,Mg2+-ATPase. Iodoacetamide and its fluorescent derivative IAEDANS specifically bind to the identical site (Cys 674) on the C-terminal half of sarcoplasmic reticulum Ca2+,Mg2+-ATPase.

synapsesocial.com/papers/6a224c433b8e99975a4ecb93https://doi.org/10.1093/oxfordjournals.jbchem.a121922
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Reactivity of sarcoplasmic reticulum ATPase with iodoacetamide spin-label: evidence for two conformational states of the substrate binding site1982 · 26 citations
  2. 2Energy Transfer between Fluorescent Dyes Attached to Ca2+,Mg2+-ATPase in the Sarcoplasmic Reticulum11983 · 11 citations
  3. 3Patterns of proteolytic cleavage and carbodiimide derivatization in sarcoplasmic reticulum adenosine triphosphatase1988 · 37 citations
  4. 4Sarcoplasmic reticulum ATPase. Spin labeling detection of ligand-induced changes in the relative reactivities of certain sulfhydryl groups1978 · 64 citations
  5. 5Independent flexible motion of submolecular domains of the calcium-magnesium ATPase of sarcoplasmic reticulum measured by time-resolved fluorescence depolarization of site-specifically attached probes1989 · 11 citations