June 22, 1982Biochemistry

Reactivity of sarcoplasmic reticulum ATPase with iodoacetamide spin-label: evidence for two conformational states of the substrate binding site

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Population

Sarcoplasmic reticulum ATPase

Comparison

Labeling with iodoacetamide spin probe… vs Labeling in the presence of Ca2+ alone

Design

Preclinical

Authors

CCCarol CoanUniversity of the Pacific SKSusan M. KeatingRoyal Hobart Hospital

Discussion

Member takes

Implication

Reveals SERCA conformational duality under dual ligation; leaves open relevance to cardiac Ca2+ handling or failure.

Structured PICO

Population

Sarcoplasmic reticulum ATPase

Intervention

Labeling with iodoacetamide spin probe N-(1-oxy-2,2,6,6-tetramethyl-4-piperidinyl)iodoacetamide in the presence of Ca2+ and substrate

Comparator

Labeling in the presence of Ca2+ alone

Outcome

Labeling kinetics and conformational states of the substrate binding sitesurrogate

The study provides evidence for the coexistence of two conformational states of the sarcoplasmic reticulum ATPase binding pocket when both substrate and Ca2+ are bound.

Cite This Study

Coan et al. (1982) studied this question.

synapsesocial.com/papers/6a885e12054eefc4d7770415 https://doi.org/10.1021/bi00256a028

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Reactivity of sarcoplasmic reticulum ATPase with iodoacetamide spin-label: evidence for two conformational states of the substrate binding site

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Reactivity of sarcoplasmic reticulum ATPase with iodoacetamide spin-label: evidence for two conformational states of the substrate binding site

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider