Key result
Delayed rectifier K channels in human neuroblastoma SH-SY5Y cells, identified as Kv3.1 channels, activated at potentials positive to -20 mV and were sensitive to TEA and 4-aminopyridine.
Findings remain preclinical; leaves open Kv3.1 targeting in neuroblastoma.
Biophysical properties of delayed rectifier K channels in the human neuroblastoma SH-SY5Y were established using patch clamp recordings. The whole cell K+ conductance activated at membrane potentials positive to -20 mV. The midpoint of current activation was 9.6 +/- 5.1 mV, the equivalent charge was 3.7 +/-.6. Whole-cell currents inactivated slightly with time constants of 700 ms and 5 s. The K+ currents were sensitive to micromolar concentrations of TEA and 4-aminopyridine. RT-PCR experiments amplified a cDNA fragment specific for human Kv3.1 channels. Activation gating parameters in outside-out patches were shifted by approximately 14 mV in the hyperpolarizing direction.
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Friederich et al. (2003) studied Human neuroblastoma. Delayed rectifier K channels in human neuroblastoma SH-SY5Y cells, identified as Kv3.1 channels, activated at potentials positive to -20 mV and were sensitive to TEA and 4-aminopyridine.
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