March 1, 1993Journal of Neurochemistry

Stable Expression and Regulation of a Rat Brain K+ Channel

View Full Paper

Ask AI

Bookmark

Population

Human embryonic kidney cells stably transfected with the Shaw-type K+ channel Kv3.1

Comparison

Stable expression of Kv3.1 and treatment with… vs Kv3.1 channels expressed transiently in Xenopus…

Design

Preclinical

Authors

SCStuart D. CritzUniversity of South Alabama BWBarbara A. WibleMetroHealth HLHéctor S. LópezUniversidad Nacional de Córdoba

Discussion

Member takes

Implication

Supports stable HEK Kv3.1 expression as a model for channel regulation; extends oocyte data but leaves open native tissue translation.

Structured PICO

Population

Human embryonic kidney cells stably transfected with the Shaw-type K+ channel Kv3.1

Intervention

Stable expression of Kv3.1 and treatment with protein kinase C activator phorbol 12,13-dibutyrate

Comparator

Kv3.1 channels expressed transiently in Xenopus oocytes

Outcome

Biophysical properties (voltage dependence of activation, K+ permeability, sensitivity to external tetraethylammonium, unitary conductance) and regulation by phorbol 12,13-dibutyratesurrogate

Stable expression of voltage-gated K+ channels in human embryonic kidney cells is a suitable model for analyzing biophysical and biochemical regulatory processes.

Cite This Study

Critz et al. (1993) studied this question.

synapsesocial.com/papers/6a7e1401fc4f0e65bd3bfa7f https://doi.org/10.1111/j.1471-4159.1993.tb03273.x

View Full Paper

Ask AI

Bookmark

Stable Expression and Regulation of a Rat Brain K+ Channel

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Stable Expression and Regulation of a Rat Brain K+ Channel

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider