Nucleotide sequence of cDNA for murine myeloperoxidase

A cDNA encoding murine myeloperoxidase (MPO) was cloned and sequenced from a PUC19 library derived from a hemopoietic progenitor cell line induced to differentiate into promyelocytes (1). The sequence of the longest cDNA clone (mMP06) has 85% homology to the human cDNA sequences (2,3,4). At the 5' end, the first 500 nucleotides of the cDNA do not show homology to the human cDNA sequences or to the human 1420 gene sequence up to the identified initiation of transcription site (5). They are homologous (72%) to human DNA sequences immediately 5' and up to 1kb upstream of the initiation of transcription site. Five other independent murine MPO cDNA clones initiated at different sites but all within the first 500 bp of the longest clone indicating that the sites of initiation of transcription of the mouse MPO gene are further upstream than that of the corrresponding human gene. At the 3' end of the murine cDNA there is no homology to the human cDNAs or genomic clones downstream of the TAG codon. Brackets indicate regions coding for light and heavy subunits of 1420.