Key result
In silico screening identified three Tinospora cordifolia-derived compounds with binding affinities ≤ -6 kcal/mol that may act as potential inhibitors of the ASFV B175L protein.
Why the study?
Effective African swine fever virus inhibitors remain unidentified, making the viral immune-evasion protein B175L a promising target for antiviral drug development.
In silico analysis identified three compounds from Tinospora cordifolia as potential inhibitors of the ASFV B175L protein, warranting further validation.
Answers are grounded in this paper and cite their sources.
Hypothesis-generating for ASFV B175L inhibition; experimental validation required before any therapeutic consideration.
African swine fever virus (ASFV) is a highly lethal DNA virus that suppresses the host’s immune response by establishing infection. B175L, one of its key immune-evasion proteins, directly inhibits STING-mediated type I interferon (IFN-I) signalling, thereby preventing the activation of antiviral defences. Thus, targeting B175L could be a promising strategy for antiviral drug development as effective ASFV inhibitors remain unidentified. In this study, we investigated the potential of Tinospora cordifolia ’s bioactive compounds to disrupt B175L’s function and restore immune signalling. Gas chromatography-mass spectrometry (GC-MS) analysis of the methanol extract from T. cordifolia stems identified 86 compounds. These were filtered using SwissADME, ProTox 3.0, and DataWarrior, yielding 15 compounds with favourable drug-likeness and safety profiles. We generated a highly accurate 3D model of ASFV B175L with strong confidence scores for the structural accuracy, using AlphaFold3. The filtered compounds were then subjected to virtual screening with PyRx 0.8, and the 3 compounds with binding affinities ≤ −6 kcal/mol were selected for subsequent analysis. Molecular dynamics (MD) simulations were used to assess binding stability, including root mean square deviation and fluctuation (RMSD, RMSF), protein-ligand contacts, radius of gyration (rGyr), and solvent accessible surface area (SASA) using Schrödinger Maestro. Taken together, these in silico results suggest that T. cordifolia- derived Benzaldehyde, 5-bromo-2-hydroxy-, (5-trifluoromethyl-2-pyridyl) hydrazone, Carbamic acid, N-(3-oxo-4-isoxazolidinyl)-, benzyl ester, and 1H-Indol-5-ol may act as potential inhibitors of B175L and represent preliminary antiviral hits against ASFV, warranting further in vitro and in vivo validation.
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Karunarathne et al. (2026) studied African swine fever virus (ASFV). Tinospora cordifolia bioactive compounds was evaluated on Binding affinity and stability to ASFV B175L. In silico screening identified three Tinospora cordifolia-derived compounds with binding affinities ≤ -6 kcal/mol that may act as potential inhibitors of the ASFV B175L protein.
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