What question did this study set out to answer?

This research aims to explore how ADP-ribosylation by exoenzyme C3 modifies the function of the G protein p21.bot.

Functional Modification of a 21-Kilodalton G Protein when ADP-Ribosylated by Exoenzyme C3 of Clostridium botulinum

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This research aims to explore how ADP-ribosylation by exoenzyme C3 modifies the function of the G protein p21.bot.
Purified exoenzyme C3 was introduced into cells to ADP-ribosylate p21.bot.
Morphological changes in NIH 3T3 and PC12 cells were observed post-treatment.
Effects on germinal vesicle migration were assessed in Xenopus oocytes.
ADP-ribosylation led to temporary morphological changes in cells, similar to ras protein effects.
Injection of C3 in Xenopus oocytes caused germinal vesicle migration and enhanced breakdown by progesterone.
p21.bot is immunologically distinct from p21ras, indicating functional differences.

Randomized trial investigates how exoenzyme C3 alters G protein functions, suggesting new pathways for cell signaling.

Rubin et al. (1988) studied this question.

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