Key result
DAPT is linked to ~32% lower PF4 levels vs aspirin alone, correlating with 24-hour bleeding.
Why the study?
Managing bleeding risk during cardiac surgery requires understanding platelet dynamics, especially when antiplatelet agents are maintained until CABG, prompting exploration of plasma sCD40L, sP-Selectin, PF4, and NAP-2 for predicting bleeding.
Do plasma levels of PF4 and NAP-2 predict postoperative bleeding in patients undergoing CABG on antiplatelet therapy?
Observational (n=143)
Do plasma levels of PF4 and NAP-2 predict postoperative bleeding in patients undergoing CABG on antiplatelet therapy?
Plasma PF4 quantification could provide a simple alternative to aggregation tests for predicting platelet reactivity and postoperative bleeding to guide perioperative antiplatelet management in CABG patients.
PF4 levels may aid bleeding prediction in CABG on DAPT; hypothesis-generating and should not yet change practice.
Platelet activation is crucial for hemostasis and thrombosis, particularly in patients on antiplatelet therapy. Managing bleeding risk during cardiac surgery requires understanding platelet dynamics, especially when antiplatelet agents are maintained until the coronary artery bypass surgery (CABG). to explore the relevance of plasma levels of sCD40L, sP-Selectin, PF4, and NAP-2 in predicting bleeding events during CABG in patients under dual antiplatelet therapy (clopidogrel+aspirin, DAPT) or aspirin. Platelet reactivity before CABG was assessed in 143 patients by plasma levels of sCD40L, sP-Selectin, PF4, and NAP-2 as well as platelet aggregation tests and platelet membrane expression of P-Selectin. Postoperative bleeding was monitored at 24 h with the chest tube blood output. Patients receiving DAPT demonstrated markedly reduced plasma levels of PF4 and NAP-2 compared to patients on aspirin alone (PF4: 676 vs. 992 ng/ml, p < 0.001; NAP-2: 167 vs. 281 ng/ml, p < 0.001). PF4 and NAP-2 levels correlated with 10 and 20 µM ADP-induced and collagen-induced platelet aggregation ( p = 0.01) but not with arachidonic acid or PAR1ap as agonists. Platelet membrane P-Selectin expression before CABG was significantly correlated with PF4 and NAP-2 levels ( r = 0.26, p < 0.001 for PF4, r = 0.23, p = 0.01 for NAP-2), confirming its role in reflecting basal platelet activation. sP-Selectin and sCD40L showed no significant correlation with any clinical outcomes, treatment group or platelet activation test whereas PF4 and NAP-2 were independently correlated with increased 24-hour bleeding volume (PF4: r =-0.19 p = 0.03; NAP-2: r =-0.17 p = 0.04). PF4 is the most platelet-specific biomarker for predicting platelet reactivity and bleeding in patients receiving DAPT until the CABG. Plasma PF4 quantification could provide a simpler alternative to aggregation tests for predicting platelet reactivity, postoperative bleeding and could guide perioperative antiplatelet management.
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Bezieux et al. (2026) conducted an observational in Coronary artery bypass surgery (CABG) (n=143). DAPT (clopidogrel+aspirin) vs. Aspirin alone was evaluated on Plasma PF4 levels (ng/ml) (p=<0.001). Patients receiving DAPT had significantly lower plasma PF4 levels compared to aspirin alone (676 vs 992 ng/ml, p<0.001), and PF4 levels independently correlated with 24-hour bleeding volume.
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