Experiments on Growth Media for Lactobacilli

Summary: A medium permitting consistently good growth of known strains of lactobacilli was determined using nineteen strains considered difficult to grow. For the culture media tested various concentrations of albumin, starch, Tween 80, sodium oleate and Yeastrel were added to tomato dextrose broth and strain growth was measured by a photo‐electric absorptiometer. Statistical analysis showed that, of the combinations tested, the best response was achieved by the addition to tomato dextrose broth of Tween 80 (0.1% v/v) and Yeastrel (0–6% w/v) with or without 0.5% of starch.