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The separation of triglycerides by reversed-phase partition chromatography in columns is described. Heptane on nonwetting Celite is used as the stationary phase and acetonitrile-methanol as the mobile phase. Mixtures of triglycerides differing by only one methylene group or one double bond are well resolved. The method may be used for the separation of triglycerides containing cis and trans unsaturated fatty acids that cannot be separated by either gas-liquid or argentation chromatography.
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Nickell et al. (1967) studied this question.
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