December 1, 1984Biochemistry

Use of gated perfusion to study early effects of anoxia on cardiac energy metabolism: a new phosphorus-31 NMR method

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Structured PICO

Population

Isolated, perfused working rat heart

Intervention

Gated 31P NMR measurements during alternating perfusion with aerobic (95% O2/5% CO2) and anoxic (95% N2/5% CO2) medium (4.5-s O2 and 1.5-s N2 perfusions)

Comparator

Full aerobic perfusion

Outcome

Rapid changes in intracellular levels of phosphorus-containing compounds (Pi, ATP, creatine phosphate, NAD(H)) and intracellular pHsurrogate

A novel gated 31P NMR method enables the detection of rapid, sub-second changes in cardiac energy metabolism during anoxia in isolated rat hearts.

Discussion

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Implication

Detects sub-second pH and energy shifts in anoxic rat hearts; leaves open whether gated 31P NMR translates to clinical ischemia.

Cite This Study

Barbour et al. (1984) studied this question.

synapsesocial.com/papers/6a22b8edcce2ba38c0cd6964 https://doi.org/10.1021/bi00320a024

View Full Paper

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Use of gated perfusion to study early effects of anoxia on cardiac energy metabolism: a new phosphorus-31 NMR method

Structured PICO

Discussion

Implication

Cite This Study

Use of gated perfusion to study early effects of anoxia on cardiac energy metabolism: a new phosphorus-31 NMR method

Structured PICO

Discussion

Implication

Cite This Study

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