Key result
Normothermic CPB does not alter brain and heart high energy phosphates or intracellular pH in sheep.
Why the study?
Does normothermic cardiopulmonary bypass alter cerebral and myocardial metabolism in an adolescent sheep model?
Does normothermic cardiopulmonary bypass alter cerebral and myocardial metabolism in an adolescent sheep model?
Normothermic cardiopulmonary bypass does not alter high energy phosphate levels or intracellular pH in the brain and heart in a sheep model, demonstrating the feasibility of using 31P NMR to study metabolism during CPB.
The development of a large animal preparation using 31P nuclear magnetic resonance (NMR) spectroscopy for the study of cerebral and myocardial metabolism during cardiopulmonary bypass (CPB) is reported. The effect of normothermic CPB on myocardial and cerebral metabolism was evaluated. Adolescent sheep were used which have low levels of 2,3-diphosphoglycerate, a compound which can interfere with the calculation of intracellular pH and inorganic phosphate content. CPB was performed using standard procedures modified for the presence of a high magnetic field and limited access to the animal. High quality 31P NMR data were obtained from the brains and hearts of these animals before and during normothermic CPB. These results demonstrate that the initiation of normothermic CPB does not change high energy phosphate levels or intracellular pH. In particular, the decreased myocardial oxygen demand associated with CPB is not associated with improvement in the levels of adenosine triphosphate or phosphocreatine. The measurements of energy metabolism and intracellular pH of the brain and heart during CPB were possible within the constraints of the NMR experiment without compromising the CPB procedure. Combining NMR and CPB techniques permits future studies of cerebral and myocardial metabolism, especially those relating to ischemia.
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Swain et al. (1990) studied Cardiopulmonary bypass. Normothermic cardiopulmonary bypass vs. Before cardiopulmonary bypass was evaluated on High energy phosphate levels and intracellular pH in brain and heart. Initiation of normothermic cardiopulmonary bypass in adolescent sheep did not change high energy phosphate levels or intracellular pH in the brain and heart.
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