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March 1, 1994Journal of Biological ChemistryOpen Access

Molecular cloning, structure, and chromosomal localization of the human inducible nitric oxide synthase gene.

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Authors

NCNicole ChartrainMerck & Co., Inc., Rahway, NJ, USA (United States)DGDavid A. GellerNorthwestern UniversityPKPatrick P. KotyWake Forest University

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Implication

iNOS gene mapping on 17cen-q11.2 may guide targeted inflammation research; leaves open clinical translation pending functional studies.

Key Points

  • To isolate and characterize the human inducible nitric oxide synthase (iNOS) gene and its chromosomal location.
  • Isolated human iNOS using overlapping cosmid clones from a genomic library.
  • Mapped transcriptional initiation site via primer extension analysis of human hepatocyte RNA.
  • Conducted PCR analysis and fluorescent in situ hybridization to determine chromosomal location.
  • The human iNOS gene is approximately 37 kilobases long, with 26 exons and 25 introns.
  • The transcriptional initiation site was mapped 30 base pairs downstream of a TATA sequence.
  • The iNOS gene is located on chromosome 17 at position 17cen-q11.2.

Cite This Study

Chartrain et al. (1994) studied this question.

synapsesocial.com/papers/6a22d522d980e385c19fe354https://doi.org/10.1016/s0021-9258(17)37441-0
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