Key result
Rhodamine attached to SH2 oriented differently (near 42 degrees) compared to SH1 and was not sensitive to MgADP, providing evidence for internal flexibility of S-1.
Population
Single glycerinated fibers of rabbit psoas muscle
Comparison
Labeling SH2 of S-1 with maleimidorhodamine and… vs Labeling SH1 withtetramethylrhodamine
Design
Preclinical
Authors
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Hypothesis-generating for myosin dynamics; leaves open relevance to cardiac contractility or therapies.
The study provides evidence for internal flexibility of the myosin S-1 head by demonstrating differential mobility of the SH1- and SH2-containing regions.
Miyanishi et al. (1989) studied this question. Labeling SH2 of S-1 with maleimidorhodamine vs. Labeling SH1 was evaluated on Orientation of chromophores measured by fluorescence detected dichroism. Rhodamine attached to SH2 oriented differently (near 42 degrees) compared to SH1 and was not sensitive to MgADP, providing evidence for internal flexibility of S-1.
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