October 1, 1982Biochemistry

Spatial relationship between a fast-reacting thiol and a reactive lysine residue of myosin subfragment 1

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Population

Myosin subfragment 1 (S-1)

Comparison

Specific labeling at SH1 with an energy donor… vs S-1 with SH1 free or RLR free

Design

Preclinical

Authors

Discussion

Provides structural constraints on myosin subfragment-1; leaves open functional impact on cardiac contractility.

Population

Myosin subfragment 1 (S-1)

Intervention

Specific labeling at SH1 with an energy donor (AEDANS) and at RLR with an energy acceptor (TNBS)

Comparator

S-1 with SH1 free or RLR free (singly labeled or unlabeled)

Outcome

Spatial proximity/interchromophore distance between SH1 and RLRsurrogate

The study determined the spatial distance between two key reactive residues in myosin subfragment 1 to be 2.6 nm using fluorescence energy transfer.

Takashi et al. (1982) studied this question.

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